![]() Note: The apparent molecular weight of each protein (kDa) has been determined by calibration against an unstained protein ladder in each electrophoresis condition. Sizing of proteins on SDS-polyacrylamide gels and western blots.The Prestained Protein Ladder is ideally suited for use in SDS-PAGE and western blotting. ABP Prestained Protein Marker can be used in Western blotting with all common membranes: PVDF. Monitoring protein transfer onto membranes after western blotting. Thermo Scientific Spectra Multicolor Low Range Protein Ladder is a mixture of six proteins and synthetic peptides (1.7 to 40kDa) for use as size standards for peptides and small proteins in gel electrophoresis and Western blotting.Features. (1,2) by comparison to the Protein Ladder (NEB. proteins in the ladder may migrate with the dye front. ![]() Monitoring protein migration during SDS-polyacrylamide gel electrophoresis.Membrane-compatible-colored bands transfer to membranes for Western blotting.Two reference bands-orange at 70 kDa and a mixture of blue and yellow ribbons at 10 kDa.Quality tested-each lot evaluated by SDS-PAGE and Western blotting.Sharp bands-color-coded bands of similar intensity for easy visualization.Ready-to-use-supplied in a loading buffer for direct loading on gels no need to boil.Size range-10 proteins spanning from 10 to 180 kDa.The ladder contains one orange reference band at 70 kDa and a mixture of blue and yellow ribbons at 10 kDa. ![]() This prestained protein marker is designed for monitoring the progress of SDS-polyacrylamide gel electrophoresis, for assessing transfer efficiency onto PVDF, nylon and nitrocellulose membranes, and for estimating the approximate size of separated proteins that have been made visible with gel stains or Western blot detection reagents. Compatible with chemiluminescent substrates and fluorescent secondary antibodies.ABP Prestained Protein Marker is a mixture of ten blue, orange or green-stained proteins (10 to 180 kDa) for use as size standards in protein electrophoresis (SDS-PAGE) and western blotting. The protein marker is supplied in a ready-to-use format for direct loading onto gels no need to heat, reduce, or add sample buffer prior to use.
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